1561-4921 1735-8086 jalali 1388 10 15 gregorian 2010 1 5 11 4 online 1 fulltext

1561-4921 1735-8086 The purpose of this is to identify the number of scientific papers written about stem cells by Iranian researchers and in this regard to use the results for further stem cell research by Iranian scientists. In this research we have used scientometric method is a single quantitative method. The statistical population of this article includes all articles published by Iranian researchers from the earliest records until the end of 2007 as listed in the ISI database which is the web based version of science citation index (SCI). The results show that A. Ghavamzadeh with 19 articles is the most productive Iranian researcher in the ISI database. The majority of published articles have been written by more than one author. A review of the findings show that Iranian researchers have been successful in stem cell production. Stem Cells ISI Database Publications 456 458 Rahim Alijani M.Sc. alijanir@pnu.ac.ir No Nooralah Karami M.A. No

1561-4921 1735-8086 Objective: In this study the effects of met-enkephalin on tumor infiltrating lymphocytes for cancer treatment in fibrosarcoma bearing mice has been evaluated. Materials and Methods: Initially splenocytes were cultured with several doses of met-enkephalin to obtain the most effective dose and treating time for the induction of CD25. Flow cytometry was used to evaluate CD25 expression. The best dose and treating time were used to stimulate tumor infiltrating lymphocytes (TILs). TILs were taken from tumors by enzymatic tissue disaggregation and purified by magnet bead cell separation in order to obtain pure CD4+ and CD8+ cells. After TILs stimulation they were re-injected into three groups of other fibrosarcoma bearing mice. The first group received only CD4+ TILs whereas the second group received only CD8+ TILs and the third group received both CD4+ and CD8+ TILs. A fourth group that served as the control group received phosphate buffered saline (PBS). The effect of this treatment on tumor volume mice survival effector cells regulatory T cells and Bcl-2 serum level were evaluated. One way ANOVA followed by the Tukey test was used to analyze data in both the experimental and control groups. P value below 0.05 was considered significant. Results: Treatment with met-enkephalin at a dose of 10-10 M for 6 hours was most effective in CD25 induction on the splenocytes of Balb/C mice. There was a significant decrease in tumor growth in both the CD8+ and CD4+ activated TILs injected groups (p Met-Enkephalin Fibrosarcoma Tumor Infiltrating Lymphocytes Bcl-2 Treg 408 417 Nematollah Khansari Ph.D. nematkhansari@yahoo.com No Abbas Ali Amini M.Sc. No Jamshid Hajati Ph.D. No Mohammad Vodjgani Ph.D. No Zahra Gheflati B.Sc. No Afshin Namdar M.Sc. No Marziyeh Holakuei M.Sc. No

1561-4921 1735-8086 Objective: A study of the effects of triploidy on some haematological indices of rainbow trout. Materials and Methods: Haematological characteristics such as red blood cell (RBC) dimensions area and volume RBC and white blood cell counts (WBC) haematocrit (Hct%) hemoglobin (Hb) mean erythrocytic hemoglobin (MEH) mean erythrocytic volume (MEV) mean erythrocytic hemoglobin concentration (MEHC) and RBC abnormalities were measured in 14 and 15 ten month old rainbow trout respectively. Results: Triploidy significantly increased all morphometric indices containing dimensions nuclear area and volume of RBCs in compare to diploid fish (p0.05). A significant reduction in Hb was observed in triploids (7.4 g/dL) when compared to diploid fish (9.2 g/dL; p Rainbow trout Oncorhynchus mykiss Haematology Polyploidy 442 447 Mohammad Reza Kalbassi Ph.D. Mohammad Reza Kalbassi Ph.D. Kalbassi_m@modares.ac.ir No

1561-4921 1735-8086 Objective: Considering the inhibitory effect of integrin-activity modulator (manganese) on the development of morphine tolerance; in this study we have tried to assess the effect of chronic administration of both morphine and manganese on the expression levels of β1 and β2 integrins in the dorsal horn of the lumbar spinal cord. Materials and Methods: Morphine tolerance was induced by intrathecal injection of morphine 15 μg/rat twice a day for five days. In order to study the effect of manganese on tolerance development; we injected manganese alone or in combination with morphine. The analgesic effect of morphine was assessed by using the tail flick test. Semi-quantitative reverse tranh1ion - polymerase chain reaction (RT-PCR) was used to assess the expression levels of β1 and β2 integrins. Results: As assessed on day 6 five days administration of morphine significantly increased the expression levels of integrins β1 and β2. Combined administration of morphine and manganese which inhibited morphine tolerance prevented the effect of morphine on integrins’ expression. Conclusion: Increased expression of integrins may be due to direct effect of chronic morphine or a negative feedback that resulted from the potent inhibitory effect of morphine on integrins’ activity. It seems that the activating of integrins via manganese in the presence of morphine can reverse feedback and consequently the effect of chronic administration of morphine on β1 and β2 integrins’ and expression. Our findings suggest a role for intracellular matrix molecules in the development of morphine tolerance and possibly other forms of synaptic plasticity. Morphine Gene Expression Integrins Manganese Synaptic Plasticity 448 455 1 Mohammad Javan Ph.D. 1 Mohammad Javan Ph.D. mjavan@modares.ac.ir No

1561-4921 1735-8086 Objective: To evaluate and compare the gene expression profiles of Ninjurin-1 and Ninjurin- 2 with the expressions of L1 family of cell adhesion molecules (NCAM-L1) neural cell adhesion molecules (NCAM) and N-cadherin during the course of neural differentiation of mouse neural stem cells (NSCs). Materials and Methods: Briefly for neural stem cell isolation the frontal part of an adult mouse brain was minced in phosphate buffered saline (PBS) and digested by an enzyme solution which contained hyaluronidase and trypsin. Isolated cells were cultured in medium supplemented by epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). After seven days primary neurospheres appeared in culture medium. After transfer to poly-L-ornithine coated dishes that contained culture medium supplemented with 1% fetal bovine serum (FBS) the primary neurospheres differentiated into neural-like and neuroglial-like cells. Differentiated cells were examined by morphological immunocytochemical and molecular evaluations. Results: Our results showed that isolated cells from the preventricular area of mouse adult brain proliferated in medium which contained EGF and bFGF and expansion of the cells continued until passage 14 without losing morphological and neurogenesis capacity. Multiple passaging confirmed the stemness nature of the isolated cells. The isolated NSCs were able to differentiate into neural-like and neuroglial-like cells after transfer to poly-L-ornithine coated dishes that contained culture medium supplemented with 1% FBS. Molecular studies for NCAM NCAM-L1 and N-Cadherin genes as well as immunocytochemical analysis for NCAM-L1 and NCAM proved the differentiation. Our data also revealed for the first time gene expression profiling of Ninurin-1 and Ninjurin-2 two novel cell adhesion molecules (CAMs) during the course of differentiation of neural stem cells. Conclusion: The induction of neural differentiation in mouse NSCs initiates the expression of NCAM-L1 NCAM and N-cadherin which can be the proof of complete neural differentiation. In addition our results indicate that the expression of Ninjurin-1 increases after neural induction much like the expressions of NCAM-L1 NCAM and N-cadherin. This data suggests the probable importance of Ninjurin-1 in both the morphology and function of neural cells. The data in this study also reveals that the expression of Ninjurin-2 in contrast with Ninjurin-1 initiates and continues until the differentiation termination point. This suggests that although Ninjurin-1 and Ninjurin-2 share conserved hy<font>drop</font>hobic regions for their transmembrane domains their roles in nerve cells are probably different. Differentiation NCAM N-cadherin Ninjurin-1 Ninjurin-2 390 399 Fardin Fathi Ph.D. Fardin Fathi Ph.D. farfath@yahoo.com No

1561-4921 1735-8086 Objective: To study the possible association between high levels of fetal haemoglobin (HbF) in β-thalassemia intermedia patients and HS-111 and 3`HS1 sequence variations. Materials and Methods: In this study the 3' HS-1 and HS-111 regions of 30 ß-thalassaemia intermedia patients (ß°/ß°) with high levels of HbF 21 ß-thalassemia major patients and 40 normal Iranian individuals were analyzed by single-strand conformation polymorphism (SSCP) and polymerase chain reaction (PCR) sequencing. Results: Two nucleotide variations in 3' HS111 (-21A>G) and 3`HS1 (179C>T) were identified. The most frequent sequence variation was 3' HS111 (-21A) in the intermedia patients and 3`HS111 (-21G) in the major thalassemia patients. In contrast to the 3`HS1 marker both 3'HS111 A and G variants showed a correlation with each studied group. Conclusion: The HS111 marker in conjunction with other parameters could be used as appropriate genetic markers to discriminate β-thalassemia intermedia patients (β°/β°) with high levels of HbF from β-thalassemia major patients. β-Thalassemia Fetal Haemoglobin Single-Strand Confirmation Polymorphism 418 423 Frouzandeh Mahjoubi Ph.D. Frouzandeh Mahjoubi Ph.D. Frouz@nigeb.ac.ir No

1561-4921 1735-8086 factor-I (IGF-I) were investigated during the ovarian developmental stages in Persian sturgeon. In addition the effects of growth hormone and thyroxine were investigated on IGF-I immunoreactivity in vitro. Materials and Methods: Ovarian samples were taken from two brood stocks (caught from the sea and from a river) during their reproductive migration and at three different developmental stages based on their polarization index (PI). The effects of two hormones on IGF-I immunoreactivity were studied at two ovarian developmental stages (PI > 0.1 and PI < 0.07). In both experiments the immunoperoxidase method was performed in order to detect immunohistochemical localization and immunoreactivity of IGF-I. Results: Immunohistochemical localizations of IGFI were detected in the follicular layers. There was no significant immunoreactivity difference between the two brood stocks however in the river brood stock IGF-I immunoreactivity was more intense in the ovaries with PI < 0.07 than of PI > 0.1 (p < 0.05). Growth hormone (10 ng/ml) increased IGF-I immunoreactivity in ovarian samples from the river brood stock when their PI was less than 0.07 however thyroxine had not such effect (p < 0.05). Conclusion: Our results showed that IGF-I is present in the ovaries of Persian sturgeon and its reactivity is different among their gonadal development stages. This may support a role for IGF-I during reproductive physiology in female brood stocks of the Persian sturgeon. Moreover growth hormone is a potential hormone to increase IGF-I immunoreactivity in the ovaries of this species. Acipenser persicus Insulin-Like Growth Factor-I Ovary Immunohistochemistry 432 441 Barzan Bahrami Kamangar Ph.D. Barzan Bahrami Kamangar Ph.D. bbkamangar@yahoo.com No

1561-4921 1735-8086 Abstract Objective: Two types of stem cells can be found in the bone marrow: hematopoietic stem cells and marrow stromal cells (MSCs). If it were possible to induce the differentiation of bone marrow stromal cells into neural cells in vitro and subsequently transplant them into the brain this might help repair lesions observed in some neurodegenerative disorders such as Parkinson’s disease (PD). Materials and Methods: In this study cultured MSCs were incubated in serum free medium containing 10-8 M selegiline for 24 hours and cells were cultured for another 48 hours in α minimal essential medium (α-MEM) containing 20% fetal bovine serum (FBS). We immunostained selegiline-treated cells for neuronal markers such as NF-200 and TH. Results: Cell counting results indicated that Selegiline at doses of 10-6 10-7 and 10-8 M in comparison to other doses increased the mean percent of viable cells. The most effective dose of Selegiline for differentiation of bone marrow stromal cells (BMSCs) was 10-8 M. Molecular study indicated that the expression BDNF GDNF NGF NT3 NT4/5 genes was increased in Selegiline-treated cells in comparison to non-treated group. Conclusion: BMSCs can be directed to a neural fate in vitro and can be considered as a cell source in neurological disorders for autograft therapy. Bone Marrow Stromal Cells Selegiline Neurotrophic Factors 400 407 Maryam Haji Ghasem Kashani Ph.D. Maryam Haji Ghasem Kashani Ph.D. kashani_tmu @yahoo.com No

1561-4921 1735-8086 Abstract Objective: cortisol is the major corticosteroid in fish osmoregulation and Persian sturgeon is one of the endangered and economical species of the Caspian Sea sturgeons; this study is one of the first to investigate the effects of cortisol on one species of the Acipenserids species. Materials and Methods: Samples were fixed in Bouin’s solution dehydrated embedded with paraplast and subsequently sectioned. Immunohistochemical studies were performed by using IgGα5 and flourescin isothiocyanate conjugated (FITC) antibodies through fluorescence light microscopy. Measurements of the chloride cells were examined by Image Tools (2.0) image analysis software. Results: In the cortisol treatment there were 492 chloride cells per mm2 of the gill epithelium which was significantly (p = 0.01) higher than the control group (289 chloride cells). The lengths of chloride cells were 13.9325 ± 0.5 μm and 16.0935 ± 0.5 μm in the cortisol and control groups respectively; as reported the length was significantly smaller in the cortisol group (p = 0.02). The widths of the chloride cells were 7.718 ± 0.3 μm and 7.922 ± 0.4 μm in the cortisol and control groups which were without any significant differences. Both the dispersion and numbers of chloride cells in four locations (on the filament basement of the lamellae interlamellar region and on the lamellae) were significantly different (p = 0.01) between the two experimental groups. Conclusion: exogenous cortisol can cause significant cellular and morphometric changes in gills of the Persian sturgeon fry for their adaptation to salinity. Na+ K+-ATPase Cortisol Gill 424 431 Zahra Khoshnood M.Sc. Zahra Khoshnood M.Sc. ZKhoshnood@gmail.com No

1561-4921 1735-8086 Angiogenesis the development of new blood vessels from existing vasculature is essential in physiological processes such as growth and development wound healing and reproduction. It is also involved in pathological conditions such as tumor growth metastases and certain chronic diseases. Angiogenesis is dependent on a delicate equilibrium between endogenous angiogenic and antiangiogenic factors. However under pathological conditions this tight regulation becomes lost which can result in the formation of the different diseases including corneal neovascularization endometriosis obesity atherosclerosis diabetic retinopathy psoriasis and cancer. In general the process of angiogenesis is a multi-factorial and highly structured sequence of cellular events comprising migration proliferation and differentiation of endothelial cells and finally vascular formation maturation and remodelling. Due to the critical role of angiogenesis in physiological and pathological conditions scientists have designed various experimental models to assay angiogenesis in vitro ex vivo and in vivo. Currently many researchers profit from these experimental models that have been created for numerous research applications such as many angiogenic and antiangiogenic factors which can be used to discover of therapeutic applications. Angiogenesis Antiangiogenic Factor Angiogenesis Model Tumor 374 381 Ali Mostafaie Ph.D Ali Mostafaie Ph.D amostafaie@kums.ac.ir No

1561-4921 1735-8086 Embryonic stem cells are characterized with two specific properties: self renewal and differentiation potential. Embryonic stem cells are pluripotent cells that can be differentiated into three kind of germ layers; ectoderm endoderm mesoderm. These properties make them ideal for developmental research toxicology and transplantation in animal model of human diseases. These cells can be differentiated spontaneously into three germ layer cells but in direct differentiation molecules and growth factors involved in natural development of desired cells must well characterized to gain a proper differentiation in vitro. There are increasing numbers of death because of liver disease and failure of organ transplantation in our country and the world. This made stem cell scientists to work on embryonic stem cell differentiation to hepatocyte like cells to create an accessible cell source in regenerative medicine of liver disease in the future and also to establish stem cell derived hepatocyte for in vitro screening of drugs. In this review we will summarize the process of liver development including molecules and growth factors incorporate in the liver development as a template for in vitro differentiation of mouse and human embryonic stem cells and then we will discuss the related studies and techniques for analyzing functionality of differentiated cells. Embryonic Development Hepatocytes Embryonic Stem Cells Differentiation 348 373 Hossein Baharvand Ph.D. Hossein Baharvand Ph.D. baharvand@royaninstitute.org No

1561-4921 1735-8086 sue counts motility viability morphology and chromatin quality of epididymal sperm as well as the testicular spermatid number (TSN) per gram of testis and daily sperm production (DSP) in L-carnitine treated mice. Materials and Methods: In the present study adult male NMRI mice (mean age of 4 weeks) were administered L-carnitine by gavage for two weeks. The experimental groups received 1mg L-carnitine/100 μl deionized water and 10 mg L-carnitine/100 μl deionized water respectively. The control group did not receive L-carnitine. All samples were assessed according to World Health Organization (WHO) criteria. Sperm morphology was assessed with papanicula staining. Sperm chromatin quality was assessed using anilineblue staining. The left testes were fixed in Bouins solution for histological examination and the end slices were stained with hematoxilin and eosin (H&E). The right testis was homogenized and TSN and DSP were calculated with an improved neubauer haemocytometer and respective formula. Results: Administration of L-carnitine induced significant reduction of body weight (p L-carnitine Spermatogenesis Testis Sperm 382 389 Zohre Zare M.Sc Zohre Zare M.Sc zare1980@gmail.com No