|
Vol:
10, No: 38, Summer 2008
|
|
Original Article |
|
Investigation of Apoptosis Induction in
Differentiated PC-12 Cells after Exposure to Hydrostatic Pressure |
|
S. Sadri, M.Sc., M. Davary Zanjani, M.Sc., M. Azadbakht, Ph.D.* , A. Amini,
Ph.D.,
M. Hil, Ph.D.
* Corresponding Corresponding Address:
P.O.Box: 67149-67346, Biology Department, Faculty of Sciences, Razi University,
Kermanshah, Iran
Email: azadbakhtm@razi.ac.ir
|
|
Received: 4/Feb/2008, Accepted: 19/Apr/2008
Objective: Hydrostatic pressure is crucial component of cell environment and
fundamental physical quantity, also it is the main factor of both cell integrity
and function. Pressure variation disorder, beyond physiological limits, may lead
to pathological states. In this study, we examined the effect of hydrostatic
pressure on apoptosis induction, viability, morphology, adhesion potency to
substrate and migration of differentiated PC-12 cells.
Materials and Methods: PC-12 as a neuronal cell line maintained in RPMI
1640 culture medium supplemented with 10% fetal bovine serum. Staurosporine was
used for differentiating of mitotic PC-12 cells to post mitotic and
differentiated neuronal cells. Exclusion Dye was used for viability assay, total
neurite length of each cell as well as morphometry. TUNEL staining was also
performed for apoptosis detection, adhesion potency of cells to substrate and
evaluation of cell migration.
Results: Hydrostatic pressure, over physiological limits, induced
apoptosis in differentiated PC-12 cells. It changed cell viability gradually and
reduction happened significantly after 24 hours (p<0.05). In compare to the
control group, hydrostatic pressure reduced total neurite length, adhesion
potency to substrate and migration of cells in the examined group (p<0.05).
Conclusion: Hydrostatic pressure induced apoptosis in differentiated
PC-12 cells as a result of inappropriate interaction between cells and
substrate. We propose that apoptosis in differentiated PC-12 cells may be an
anoikis causing to lose the attachment to the substrate.
Keywords: Hydrostatic Pressure, Apoptosis, Anoikis, Neuronal
Differentiation,
PC-12 Cells
|
|
|