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A. Jahanshahi, M.Sc., J.
Mirnajafi-Zadeh, Ph.D.*, M. Javan, Ph.D.,
M. Mohammad-Zadeh, Ph.D., R. Rohani, M.Sc.
* Corresponding Address:
P.O.Box: 14115-331, Physiology Department, School of Medical
Sciences, Tarbiat Modares University, Tehran, Iran
Email: mirnajaf@modares.ac.ir
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Received: 19/Dec/2007, Accepted: 15/Mar/2008
Objective: It has been suggested that low frequency stimulation (LFS) exerts
its inhibitory effect on epileptogenesis through adenosine receptors activation.
In the present study, effect of different LFS frequencies on A1 and A2A
receptors gene expression was investigated in perforant path kindled seizures.
Materials and Methods: Animals were kindled by perforant path
stimulation.
Afterdischarges were recorded from the dentate gyrus. LFS (0.5, 1 and 5 Hz) was
applied at the end of each kindling stimulation. On the 7th day, A1 and A2A
receptors gene expression were evaluated in the dentate gyrus.
Results: Application of different LFS frequencies retarded the kindling
acquisition. Also, it decreased the afterdischarge durations and behavioural
seizure stages 4 and 5 significantly. LFS application prevented the kindling
induced decrease in the A1 receptor gene expression. On the other hand, LFS
attenuated the level of A2A
receptor gene expression in the dentate gyrus. LFS had the most effect at the
frequency of 5 Hz.
Conclusion: It may be suggested that antiepileptogenic effects of LFS is
mediated somehow through changes in the gene expression of adenosine A1 (which
has
inhibitory effects) and A2A (which has excitatory effects) receptors. These
effects might be somehow frequency dependent.
Keywords: Low Frequency Stimulation, Adenosine Receptors,
Kindling, Dentate Gyrus |
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